Analysis of Pulsatile and Surge-like Luteinizing Hormone Secretion with Frequent Blood Sampling in Female Mice
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概要
- 論文の詳細を見る
Mice have become more important as genetically-modified model animals for analysis of physiological functions. The establishment of a frequent blood sampling system in conscious mice would provide a powerful tool for a better and more detailed understanding of the physiological status of circulating hormonal changes, such as pulse or surge modes of luteinizing hormone (LH) secretion. Frequent blood sampling, however, is considered problematic in mice because of the limited blood volume for their small body size. The present study, therefore, aims to establish a blood sampling protocol to determine the pulse and surge modes of LH secretion using intra-atrial cannulation and frequent blood sampling in free-moving conscious mice. Ovariectomized mice were bled every 3 min for 1.5 h to detect LH pulses. Blood glucose levels, an indicator of stress, were kept constant throughout the 1.5-h sampling period, suggesting that sampling can be performed under stress-free conditions. Obvious LH pulses were observed in ad lib-fed ovariectomized mice, whereas they were significantly suppressed after a 24-h fast. This indicates that the present sampling protocol is suitable for detecting physiological changes in pulsatile LH secretion. In addition, 1-h-interval blood collections in proestrous mice between 1300 and 2200 h revealed that individual preovulatory LH surges occur in the evening of proestrous days. Thus, the present study has developed a blood sampling protocol to detect individual profiles of pulse and surge modes of LH secretion in mice.
著者
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束村 博子
名古屋大学 大学院生命農学研究科生殖科学研究分野
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束村 博子
名古屋大院生命農学
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束村 博子
名古屋大学大学院生命農学研究科生命技術科学専攻
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Tsukamura Hiroko
Graduate School Of Bioagricultural Sciences Nagoya University
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UENOYAMA Yoshihisa
Graduate School of Bioagricultural Sciences, Nagoya University
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Maeda Kei-ichiro
Graduate School Of Agricultural Sciences Nagoya University
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Tamamura Hirokazu
Faculty Of Pharmaceutical Sciences Kyoto University
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Tamamura Hirokazu
Dept. Of Molecular Recognition Inst. Of Biomaterials And Bioengineering Tokyo Medical And Dental Uni
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Uenoyama Yoshihisa
Department Of Bioengineering Sciences Graduate School Of Bioagricultural Sciences Nagoya University
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MINABE Shiori
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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Uenoyama Yoshihisa
Graduate School Of Bioagricultural Sciences Nagoya University
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Minabe Shiori
Graduate School Of Bioagricultural Sciences Nagoya University
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