Subunits of Human Chorionic Gonadotropin and their Radioimmunoassays
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概要
- 論文の詳細を見る
HCG subunits were isolated from hCG by chromatography on DEAE Sephadex A-50. Iodination of hCG by the enzymatic iodination method resulted in a preparation labeled almost exclusively on the α-subunits of hCG, and masking of tyrosine residues in the β-portion when bound to the α-portion of hCG molecule was interpreted.<BR>Antisera of the hCG subunits enabled radioimmunoassay of those subunits. Sera obtained from pregnant women were radioimmunoassayed for hCG, hCG-α and hCG-β in respective homologous radioimmunoassay systems. Real amounts of those hormones were calculated by subtracting the interferes of non-homologous hormones in the homologous assays. Absolute amounts of hCG reached its peak (several microgrammes per m<I>l</I>) in the first trimester, then decreased and continued the values less than 1.5 microgrammes per m<I>l</I> thereafter. Surprisingly the amounts of hCG-α in sera increased in the former half, reaching several hundreds nanogrammes per m<I>l</I> in the latter half of pregnancy, while the amounts of hCG-β were ranged in less than 100 nanogrammes per m<I>l</I>. The significance of immunoreactive hCG-α during pregnancy was speculated.
- 社団法人 日本内分泌学会の論文
著者
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Tojo Shimpei
Department Of Gynecology And Obstetrics Kyoto University School Of Medicine
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Ashitaka Yoshihiko
Department Of Gynecology And Obstetrics Kobe University Faculty Of Medicine
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Nishimura Ryuichiro
Department Of Obstetrics And Gynecology Hyogo Medical Center For Adults
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ENDOH YOSHINORI
Department of Obstetrics and Gynecology, Kobe University School of Medicine
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Nishimura Ryuichiro
Department Of Gynecologic Oncology Hyogo Cancer Center
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