EFFECTIVE SEPARATION OF HUMAN CHORIONIC GONADO-TROPIN SUBUNITS BY HIGH PERFORMANCE GEL PERMEATION CHROMATOGRAPHY

スポンサーリンク

概要

• 論文の詳細を見る

• Human chorionic gonadotropin (hCG) was effectively separated into α and β subunits by treating with 8 M guanidine hydrochloride, followed by high perfomance gel permeation chromatography (GPO on a 2.5 x 60 cm TSK G 3000 SW column in 6 M guanidine hydrochloride.Their molecular weights estimated by GPC were about 29,000 daltons for the P subunit and 19,000 daltons for the a subunit.Both subunits were examined for SDS-polyacrylamide gel electrophoresis, amino acid analyses and radioimmunoassay, respectively. Neither subunit showed significant binding to the hCG-receptor fraction of rat Leydig cell in vitro. However, the separated subunits easily recombined and recovered about 90% of receptor binding activity of intact hCG.The present results indicate that the GPC technique is an effective, simple and non-denaturing method for the separation of hCG into α and β subunits.

• 社団法人日本産科婦人科学会の論文
• 1986-03-01

著者

• NISHIMURA Ryuichiro

  Department of Obstetrics and Gynecology, Hyogo Medical Center for Adults

• MOCHIZUKI Matsuto

  Department of Obstetrics and Gynecology, Kobe University School of Medicine

• Mochizuki Matsuto

  Department Of Obstetrics And Gnecology Kobe University School Of Medicine

• Mochizuki Matsuto

  Departmelet Of Obstetrics Aud Gynecology Kobe University School Of Medicine

• YUKI Yoshikazu

  Department of Obstetrics and Gnecology, Kobe University School of Medicine

• Yuki Y

  Jcr Pharmaceuticals Co. Ltd. Kobe Jpn

• Yuki Yoshikazu

  Department Of Mucosal Immunology The Institute Of Medical Science The University Of Tokyo

• Nishimura Ryuichiro

  Department Of Obstetrics And Gynecology Hyogo Medical Center For Adults

関連論文

• The mucosal immune system for secretory IgA responses and mucosal vaccine development
• Eradication and reinfection of human papillomavirus after photodynamic therapy for cervical intraepithelial neoplasia
• Ovarian malignant mixed mesodermal tumor producing the free form of the β-subunit of human chorionic gonadotropin
• Combination Assay of Urinary β-Core Fragment of Human Chorionic Gonadotropin with Serum Tumor Markers in Gynecologic Cancers
• Paclitaxel-cisplatin combination in advaced ovarian cancer: a phase II study
• Urinary Levels of Nitric Oxide Metabolites in Normal Pregnancy and Preeclampsia
• The Role of Nitric Oxide Metabolites during Pregnancy
• Enhanced Magnetic Resonance Imaging in Monitoring of Conservative Treatment of Cervical Pregnancy
• Immunohistochemical Evidence for the Presence of Tumor Necrosis Factor-α in the Infant and Adult Human Ovary
• Factors Affecting Prognosis of Intrauterine Hydrocephalus Diagnosed in the Third Trimester : Computerized Data Analysis on Controversies in Fetal Surgery
• HCG PRODUCTION OF NON-TROPHOBLASTIC CANCER CELL LINES AND ITS MODULATION BY SODIUM BUTYRATE
• EFFECTIVE SEPARATION OF HUMAN CHORIONIC GONADO-TROPIN SUBUNITS BY HIGH PERFORMANCE GEL PERMEATION CHROMATOGRAPHY
• DISCORDANT INDUCTION OF TUMOR-ASSOCIATED ANTIGEN, TA-4 AND CHORIONIC GONADOTROPIN β IN CULTURED CERVICAL CARClNOMA CELLS
• Chorionic Gonadotropin Synthesized in Cultivated Trophoblast
• Lipolytic Action of Human Chorionic Somatomammotropin
• Human Chorionic TSH (hCTSH, hCT) during Normal or Molar Pregnancy
• The Clinical Evaluation of the Simultaneous Measurements of Human Chorionic Gonadotropin (hCG) and its Alpha-subunit in Sera of Patients with Trophoblastic Diseases
• Alpha-Subunit of Human Chorionic Gonadotropin in Fluid of Molar Vesicles
• Subunits of Human Chorionic Gonadotropin and their Radioimmunoassays
• Serum and Chorionic Tissue Concentrations of Human Chorionic Gonadotropin and Its Subunits During Pregnancy
• MRI Findings of an Ovarian Dermoid Cyst with Malignant Transformation
• The Characterization of Alpha-Subunit of Glycoprotein Hormone Produced by Undifferentiated Carcinoma
• Questionnaire survey of the current status of radical trachelectomy in Japan
• Purification of Human Placental Lactogen and Its Clinical Application by Single Radial Immunodiffusion
• STUDIES ON THE THYROTROPIC AND GONADOTROPIC SUBSTANCES BY "PAPERGRAPHY"
• THYROTROPIN IN PLACENTA AND FETUS

もっと見る 閉じる

スポンサーリンク