Release of human chorionic gonadotropic (hCG) and its alpha-subunit (hCG-.ALPHA.) from perifused human placenta.

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概要

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• The release of human chorionic gonadotropin (hCG) and its α-subunit (hCG-α) from the normal human placenta and the effect of some stimulatory agents on their release were studied in vitro using a perifusion system. Each perifusate was assayed for hCG and hCG-α in its own homologous radioimmunoassay systems.<BR>Both hCG and hCG-α were released from the placenta at any stage of gestation in our perifusion system. Much more hCG than hCG-α was released from the placenta in early gestation. By comparison, however, hCG-α increased gradually with the gestational age. The amount of hCG-α released was almost equal to that of hCG in the placenta in the 17th gestational week. After the 22nd gestational week, hCG-α was released in larger quantities than hCG, and about 10times more hCG-α than hCG was released from the term placenta.<BR>These results were also confirmed by gel filtration of perifusates on a Sephadex G-100 column. hCG-α, compared with hCG, was present in excess in gel filtrated perifusates in the last two trimesters.<BR>By adding 1mM dibutyryl cyclic AMP to the perifusion medium, the release of both hCG and hCG-α was stimulated significantly. Synthetic luteinizing hormone releasing hormone (LH-RH) at concentrations of 10ng/m<I>l</I> and 100ng/m<I>l</I> had no effect, but at a high concentration (1μg/m<I>l</I>), LH-RH stimulated the release of them. Moreover, mouse epidermal growth factor (EGF) stimulated not only the release of hCG and hCG-α but also their production, because both hCG and hCG-α levels rose progressively with the time course in the presence of EGF.<BR>The present studies demonstrate that the perifusion system of chorionic tissues is a useful method for investigating the release of hCG and its subunits <I>in vitro</I>.

• 社団法人 日本内分泌学会の論文

著者

• Tojo Shimpei

  Department Of Gynecology And Obstetrics Kyoto University School Of Medicine

• Ashitaka Yoshihiko

  Department Of Gynecology And Obstetrics Kobe University Faculty Of Medicine

• Takemori Masayuki

  Department Of Gynecology Hyogo Medical Center For Adults

• Nishimura Ryuichiro

  Department Of Gynecologic Oncology Hyogo Cancer Center

• TAKEMORI MASAYUKI

  Department of Obstetrics and Gynecology, Kobe University School of Medicine

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• Release of human chorionic gonadotropic (hCG) and its alpha-subunit (hCG-.ALPHA.) from perifused human placenta.

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