1, 10-フェナントロリンとブロモピロガロールレッドによるカドミウムの吸光光度定量

概要

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Bromopyrogallol Red reacts with cadmium in the presence of 1, 10-phenanthroline to form a blue ternary complex. Its absorption maximum is at around 620 mμ, and the absorbance is constant over the pH range from 7.8 to 8.1. The calibration curve is linear for 20 to 240 μg/25 ml of cadmium. Variation coefficient for 100 μg of cadmium is 1.4%. The molar extinction coefficient is 10, 000. The recommended analytical procedure is as follows. Two milliliters of barbiturate buffer (pH 8.0), 2 ml of 2% acacia gum solution, 1 ml of 0.05% 1, 10-phenanthroline solution and 10 ml of 0.01% bromopyrogallol red solution are taken into a 25 ml measuring flask, and a solution containing 20 to 240 μg cadmium is added to it. It is then diluted to the mark with water and, after 15 minutes, the absorbance is measured at 610 mμ against the reagent blank. Many metal ions interfere with the determination.