イカ甲骨多糖体のゲル濾過像及び構成単糖について

概要

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The internal shell of squid was extracted with hot 10mM EDTA after being washed with acetone. The crude extract was dialyzed against water and freeze-dried. The distribution of molecular weight was determined by column chromatographies on Sephadex G-75 and Sepharose 6B, 4B. and 2B. The data suggested that the crude extract was composed of polysaccharides of widely varying molecular weights and that the molecular weight of a major component was approximately 106. After the crude extracts were acid-hydrolyzed and methanolyzed, the constituent monosaccharides were analyzed with gas chromatography. The results were as follows: L-arabinose, L-rhamnose, D-xylose, D-mannose, D-galactose, D-glucose, D-glucosamine, and D-galactosamine. Uronic acid was also detected by chemical analysis but sialic acid was undetectable.
公益社団法人日本水産学会の論文