海洋性Vibrioの生産する抗腫瘍性多糖体について-II : 多糖体の化学的および物理化学的性質(1)

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Some chemical and physicochemical properties of an antitumor polysaccharide produced by Vibrio sp. No. 11-15, were investigated. Vibrio sp. No.11-15, isolated from sea water, produced extracellular polysaccharide on cultivation in a sea water medium containing 0.5% peptone and 0.1% yeast extract for 5-7 days at 25°C. A crude preparation of the polysaccharide was obtained from the viscous fermentation broth by precipitation with acetone. The acetone-precipitated polysaccharide was broth by precipitation with acetone. The acetone-precipitated polysaccharide was redissolved in M/100 EDTA and lyophilyzed after dialysis against water. The partially purified polysaccharide preparation seemed to be homogeneous according to ultracentrifugation behavior. However, the data on cetyltrimethylammonium chloride precipitation indicated that it probably contains two different polysaccharide fractions. The chemical analysis indicated that the polysaccharide contained 11.2% carbohydrate (as mannose), 1.5% amino sugar (as glucosamine) and 34.9% protein (as bovine serum albumin). In the infrared spectrum of the polysaccharide, absorption bands around 1250 and 820cm-1 which are characteristic of ester sulfates were found. The relative viscosity [ηrel] of the polysaccharide decreased rapidly with increasing concentration of NaC1 above 0.1M. The neutralization equivalent, intrinsic viscosity and optical rotation of the polysaccharide were 972, 0.6 (g/100ml)-1 and [α]D+151° (C=0.95, water), respectively.
公益社団法人日本水産学会の論文

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奥谷康一
香川大学農学部

海洋性Vibrioの生産する抗腫瘍性多糖体について-II : 多糖体の化学的および物理化学的性質(1)

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