Surface Charge of Fractionated Guinea Pig Keratinocytes Measured by Free-flow Cell Electrophoresis.
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概要
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Keratinocytes differentiate from basal cells to spinous, granular, and horny layer cells. It is known that alterations in the surface charge of cell membranes in most cases reflect the processes of differentiation. By using a continuous colloidal silica (Percoll) density gradient, keratinocytes may be separated into three fractions which correspond to their arrangement in vivo. Using a free-flow cell electrophoretic technique, we measured the electrophoretic mobility of guinea pig keratinocytes. Electrophoretic mobility histograms of basal and granular cells showed slow and fast monophasic patterns, respectively. In spinous cells, a biphasic pattern of slow and fast electrophoretc mobility was present. The electrophoretic mobility level of guinea pig keratinocytes was slightly reduced with neuraminidase digestion. Those of human red blood cells and lymphocytes, however, were markedly decreased. These results indicate that membrane charge density is lower in basal cells and higher in granular cells and that the membrane charge density of guinea pig keratinocytes involves not only neuraminic acid residues but also other substance (s). Our results illustrate the alterations of cell membrane charge properties during epidermal cell differentiation.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Nakano Shunji
Department Of Orthopaedics Institute Of Health Biosciences University Of Tokushima Graduate School O
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Hachisuka Hiroshi
Department Of Dermatology And 2nd Department Of Pathology Kurume University
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Karashima Tadashi
Department Of Dermatology Kurume University School Of Medicine
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Mori Osamu
Department Of Applied Chemistry College Of Engineering University Of Osaka Prefecture
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Sasai Yoichiro
Department Of Dermatology And 2nd Department Of Pathology Kurume University
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Okubo Keiji
Department Of Immunology And The Third Department Of Internal Medicine Kurume University School Of M
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KUSUHARA MASAHIRO
Department of Dermatology, Kurume University School of Medicine
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OKUBO KEIJI
Department of Dermatology, Kurume University School of Medicine
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NAKANO SHUNJI
Department of Dermatology, Kurume University School of Medicine
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