Human Mononuclear Cell Antibody-Dependent Cell-Mediated Cytotoxicity Toward Red Blood Cells of Various Rh Genotypes

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概要

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• Antibody-dependent cell-mediated cytotoxicity (ADCC) mediated by human peripheral blood mononuclear cells (85 % lymphocytes and 15 % monocytes) or lymphocyte-enriched (98 % lymphocytes) effector cells against human red blood cell (HRBC) targets of various Rh genotypes, with or without papain treatment, was tested in an 18-hour <SUP>51</SUP>Cr release assay. The HRBC were sensitized with Rh<SUB>0</SUB>GAM, an anti-Rh<SUB>0</SUB> (D) IgG fraction. Mononuclear cell effectors were found to lyse papain treated Rh<SUB>0</SUB> (D) homozygous cells to a greater degree than Rh<SUB>0</SUB> (D) heterozygotes. The genotypes of the HRBC targets, in order of the amount of lysis from highest to lowest was R<SUB>1</SUB>R<SUB>1</SUB>, R<SUB>2</SUB>R<SUB>2</SUB> or R<SUB>1</SUB>R<SUB>2</SUB>, R<SUB>2</SUB> r, R<SUB>1</SUB>r, R<SUB>0</SUB>r, rr. When the target cells were not papain treated and mononuclear cell effectors were used, the amount of lysis was low and the % specific lysis for Rh<SUB>0</SUB> (D) homozygote and heterozygote HRBC was equivalent. Lymphocyteenriched effector cells did not lyse the homozygous target cells to a greater degree than the heterozygotes, although the amount of lysis for both types of HRBC was greater than that noted using mononuclear cells as effectors.

• 久留米大学医学部 The Kurume Medical Journal 編集部の論文

著者

• OKUBO KEIJI

  Department of Dermatology, Kurume University School of Medicine

• OKUBO KYOICHI

  Department of Pathology and Microbiology, University of Illinois at the Medical Center Chicago

• JABS A.

  Rush Medical College

• Okubo Keiji

  Department of Applied Chemistry, Faculty of Engineering, Yokohama National University

• OKUBO KEIJI

  Department of Immunology, Kurume University School of Medicine

• OKUBO KYOICHI

  Department of Ophthalmology University of Occupational and Environmental Health

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