Dihydropyrimidine Dehydrogenase Activity and Thymidylate Synthase Level Are Associated with Response to 5-fluorouracil in Human Colorectal Cancer.
スポンサーリンク
概要
- 論文の詳細を見る
In the recent studies associated with the modulation of 5-fluorouracil (5-FU) and the development of new antifolates, attentions have been focused on the expression of the target enzymes, thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD), that affect tumor sensitivity and resistance to drugs. In order to evaluate predictability of therapeutic efficacy by intratumoral enzyme activity, we investigated the role of TS content and DPD activity in tumor sensitivity to 5-FU. Surgical specimens were obtained from 51 patients with colorectal cancer and used to measure TS content and DPD activity. TS content and DPD activity in tissues were measured by [<SUP>3</SUP>H]-FdUMP binding assay and radioenzymatic assay, respectively. The sensitivity to 5-FU in tumor specimens was determined by collagen-gel droplet embedded-drug sensitivity test (CDDST). The TS content and DPD activity did not correlate with Dukes' staging. There was no correlation between TS content and DPD activity in any tumors. Simple linear regression analysis showed that neither DPD activity (r=-O.267, p>0.05) nor TS content (r=-O.277, p> 0.05) in tumors had a significant correlation with 5-FU effectiveness independently. Four out of 24 patients, highly responsive to 5-FU, showed low levels in both DPD and TS. The patients with high value in either DPD activity or TS content proved not to respond to 5-FU. In conclusion, these results demonstrate that both tumor DPD activity and TS content are the factors predicting 5-FU responsiveness in colorectal cancer.
著者
-
Araki Yasumi
Department Of Surgery Kurume University Medical Center
-
Shirouzu Kazuo
Department Of Surgery Kurume University
-
ISOMOTO Hiroharu
Department of Surgery, Kurume University Medical Center
関連論文
- Surgery and Clinicopathological Features of Gastric Adenocarcinoma Involving the Esophago-Gastric Junction
- Long-Term Survival Following Treatment with Antineoplastons for Colon Cancer with Unresectable Multiple Liver Metastases : Report of a Case
- Axillary Lymph Node Recurrence of Papillary Thyroid Microcarcinoma : Report of a Case
- Immunological evaluation of CTL precursor-oriented vaccines for advanced lung cancer patients
- A case of benign bile duct stricture causing difficulty in differential diagnosis from bile duct carcinoma
- Nonparasitic solitary huge liver cysts causing intracystic hemorrhage or obstructive jaundice
- Prognostic Factors after Potentially Curative Resection in Stage II or III Colon Cancer
- Preclinical studies of molecular-targeting diagnostic and therapeutic strategies against breast cancer
- Adenomyoepithelioma of the Breast Diagnosed by a Mammotome Biopsy : Report of a Case
- Changes in A-type Natriuretic Peptide and Its Receptors Induced by a Neutral Endopeptidase Inhibitor in a Rat Model of Sepsis
- Vasoactive Peptides in a Pulmonary Embolism Model
- An Experimental Study of Atrial Natriuretic Peptide Levels and the Effect of Inhaled Nitric Oxide After Pneumonectomy
- Natriuretic Peptides in the Lung Modulated by Pneumonectomy
- Intraoperative ultrasonography is useful for diagnosing pancreatic duct injury and adjacent tissue damage in a patient with penetrating pancreas trauma
- Cronkhite-Canada Syndrome Associated with Advanced Rectal Cancer Treated by a Subtotal Colectomy : Report of a Case
- A clinical overview of familial adenomatous polyposis derived from the database of the Polyposis Registry of Japan
- The Influence of Pylorus-Preserving Partial Gastrectomy on Carcinogenesis of Residual Gastric Mucosa in the Rat
- Thoracoscopic Resection of a Giant Leiomyoma of the Esophagus with a Mediastinal Outgrowth
- Primary Hemangiopericytoma of the Superior Mediastinum : A Case Report
- Resection of Malignant Fibrous Histiocytoma Invading the Thoracic Aorta
- Amyloid Tumor in the Anterior Mediastinum : Report of a Case
- Incidental Partial Anomalous Pulmonary Venous Connection in Left Lung Cancer
- The UPAO Test in Preoperative Evaluation for Major Pulmonary Resection : An Operative Case with Markedly Improved Ventilatory Function after Radical Pulmonary Resection for Lung Cancer Associated with Pulmonary Emphysema
- Outcome for Malignant Tracheobronchial Stenoses in Esophageal Cancer
- Physiological Study of Anal Sphincteric Resection in an Experimental Porcine Model
- Inhibition of Liver Metastasis from Orthotopically Implanted Colon Cancer in Nude Mice by Transfection of the TIMP-1 Gene into KM12SM Cells
- Long-term Survival after Three-field Lymphadenectomy for an Adenocarcinoma in Barrett's Esophagus with Metastasis to Virchow's Node
- Evaluation of an Aortic Stent Graft for Use in Surgery on Esophageal Cancer Involving the Thoracic Aorta : Experimental Study
- Spontaneous Regression in Small Cell Esophageal Carcinoma
- Locoregional adoptive immunotherapy resulted in regression in distant metastases of a recurrent esophageal cancer
- Three-field Dissection for Squamous Cell Carcinoma in the Thoracic Esophagus
- The singnificance of telomerase activity in cancer lesions and the noncancerous epithelium of the esophagus
- Retrospective investigation of pulmonary resection in patients with high total pulmonary vascular resistance during preoperative unilateral pulmonary artery occlusion
- Selection of Pulmonary Resection Procedures to Avoid Postoperative Complications
- Expired Gas Analysis During Exercise Testing Pre-pneumonectomy
- Preoperative Spirometry Versus Expired Gas Analysis During Exercise Testing as Predictors of Cardiopulmonary Complications After Lung Resection
- Relationship Between Microvessel Density and Thermographic Hot Areas in Breast Cancer
- Biological features of sporadic colorectal carcinoma with high-frequency microsatellite instability : special reference to tumor proliferation and apoptosis
- Expression of vascular endothelial growth factor and its receptor KDR (kinase domain-containing receptor)/Flk-1 (fetal liver kinase-1) as prognostic factors in human colorectal cancer
- Laparoscopic Intraperitoneal Repair of Postoperative Ventral Incisional Hernia Using ComposixTM Mesh
- Development and validation of a modified fecal incontinence quality of life scale for Japanese patients after intersphincteric resection for very low rectal cancer
- The TIMP-1 gene transferred through adenovirus mediation shows a suppressive effect on peritoneal metastases from gastric cancer
- A Multicenter Phase II Clinical Study of Oxaliplatin, Folinic Acid, and 5-Fluorouracil Combination Chemotherapy as Second-Line Treatment for Advanced Colorectal Cancer : A Japanese Experience
- Clinicopathologic Study of Perineural Invasion in Rectal Cancer
- Changes in the Pleural Cavity by Pleurodesis Using Talc or OK-432 : An Experimental Study
- Transsacral Rectopexy for Recurrent Complete Rectal Prolapse
- Functional Results of Colonic J-Pouch Anastomosis for Rectal Cancer
- Inhibition of Acute Rejection After Skin or Cardiac Transplantation by Administration of Donor Antigens in the Rat
- Nonmalignant pericardial effusion associated with thymic cancer
- The Benefit of Post-Operative Adjuvant Chemotherapy Using Oral Fluoropyrimidines in Rectal Cancer
- Suppressive Effect of Bevacizumab on Peritoneal Dissemination from Gastric Cancer in a Peritoneal Metastasis Model
- Characteristics of recurrence after curative resection for T1 colorectal cancer : Japanese multicenter study
- Selection of Pulmonary Resection Procedures to Reduce Postoperative Complications in 200 Patients
- Esophagectomy for Thoracic Esophageal Cancer with a Double Aortic Arch : Report of a Case
- A Case of Gallbladder Cancer Associated with Pancreaticobiliary Maljunction
- Factors Related to an Early Restoration of Exercise Capacity After Major Lung Resection
- A Study on Polyethylene Glycol Electrolyte Lavage Solution and Sodium Picosulf ate Combined Pretreatment Method for Colonoscopy
- A Case of Pulmonary Embolism after Abdominal Angiography
- Comparison between the Gastric Cancer Cell Line MKN-45 and the High-Potential Peritoneal Dissemination Gastric Cancer Cell Line MKN-45P
- Giant Liposarcoma Occupying Most of The Hemi-Thorax and Resected in the Supine Position: Report of a Rare Case
- The Expression of p53, p21 and TGF .BETA.1 in Gastric Carcinoma
- .ALPHA.-Fetoprotein-Producing Early Gastric Cancer: Report of Two Cases
- Two Cases of Early Gastric Carcinoma with Synchronous Liver Metastasis
- Giant Malignant Phyllodes Tumor: A Case Report
- A Case of Hilar Bile Duct Cancer with Intestinal Behcet's Disease
- Circular Stapling Procedure for Mucosal Prolapse of the Rectum Associated with Outlet Obstruction.
- The Usefulness of Restorative Laparoscopic-Assisted Total Colectomy for Ulcerative Colitis.
- The Fungal DMA Examination Is Useful as a Sensitive Parameter for the Initiation and the Quit of Antifungal Therapy in Immunocompromised Pediatric Patients after Surgery
- Clinicopathological Characteristics of Colorectal Submucosal Carcinoma with Lymph Node Metastasis.
- Thymolipoma: Clinical and Pathological Features. Report of Three Cases and Review of Literature.:Report of Three Cases and Review of Literature
- Rapid-growth Lung Cancer Associated with a Pulmonary Giant Bulla: A Case Report
- Bilateral Pleural Effusion following Cervical Abscess Drainage. A Case Report.
- Improvement of Dysgeusia after Thymectomy with Thymoma.
- Two Cases of Cancer in the Remnant Stomach Derived from Gastritis Cystica Polyposa.
- Morphological Change in the MNNG-Treated Rat Gastric Mucosa.
- Advanced Gastric Cancer Effectively Treated by Neoadjuvant Continuous Low-dosage 5-fluorouracil and Cisplatin(FP Regimen): A Case Report.:A Case Report
- The B-Wave Bipolar Snare for Endoscopic Treatment.
- Mast Cells and Histamine Release in Crohn's Disease.
- Submucosal Gastric Cancer with Lymph Node Metastasis. A Clinicopathological Study.:A Clinicopathological Study
- Clipless Hand-assisted Laparoscopic Total Colectomy Using LigaSure Atlas
- Graciloplasty for Internal and External Sphincteric Resection of Lower Rectal Cancer
- An In Vitro Chemosensitivity Test for Colorectal Cancer Using Collagen-gel Droplet Embedded Cultures.
- Early Gastric Carcinoma Associated with Amyloidosis: A Case Report.
- Evaluation of the Epidermal Growth Factor Receptor (EGFR) and c-erbB-2 in Superspreading-type and Penetrating-type Gastric Carcinoma.
- Primary Gastric Lymphoma. A Clinicopathological Study.
- Clinical Examination of the Water-Soluble Vitamin Levels in Blood during Peripheral Parenteral Nutrition
- Endoluminal Ultraflex Stent for Palliative Treatment of Malignant Rectosigmoidal Obstruction.
- Clinical Aspects of Total Colectomy. Laparoscopic Versus Open Technique for Familial Adenomatous Polyposis and Ulcerative Colitis.:Laparoscopic Versus Open Technique for Familial Adenomatous Polyposis and Ulcerative Colitis
- Endoscopic Removal with Clipping for Colonic Lipomas.
- Total Mesorectal Excision, Lateral Lymphadenectomy and Autonomic Nerve Preservation for Lower Rectal Cancer: Significance in the Long-term Follow-up Study.:Significance in the Long-term Follow-up Study
- Therapeutic Rectoscopy for Rectal Tumors.
- Clinicopathological Studies of Multiple Cancers in the Large Bowel and Other Organs.
- Transanal Dilation Using Circular Stapling for Benign Rectal Stenosis: Report of a Case.
- Dihydropyrimidine Dehydrogenase Activity and Thymidylate Synthase Level Are Associated with Response to 5-fluorouracil in Human Colorectal Cancer.
- Function Outcome of Double-Stapled and Transanal Ileal Pouch-Anal Anastomosis after Proctocolectomy.
- A Case of Argon Plasma Coagulation Therapy for Hemorrhagic Rectal Tumor in a Highly Aged Patient
- Transsacral Rectopexy for Complete Rectal Prolapse.
- Occurrence of Desmoid Tumors following Surgery for Familial Adenomatosis Coli. Report of Two Cases.:Report of Two Cases
- Survival of rectal carcinoma patients studied with flow cytometric DNA analysis.
- Intraductal Ultrasonography for Treatment of Tis and T1 Colorectal Cancer.