Immunocytochemical study of ras oncogene product p21 in liver cirrhosis and hepatocellular carcinoma.

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Immunocytochemical assay to assess expression of ras gene product p21 in normal liver, liver cirrhosis (LC) and hepatocelMar carcinoma (HCC) of the autopsied cases was performed by avidin-biotin-peroxidase method. With the concentration of monoclonal antibody used in this study (RAP-5 given by Dr. J. Scholm, NIH Bethesda MD), hepatocytes from normal liver were all negative for p21 antigen. This antigen in regenerative nodules was detected in 89% (59/66)of LC with HCC, wkereas 50% (9/18) of LC without HCC (p<0.005). In LC without HCC incidence of p21 expression was tended to be higher in hepatic HBV-positive than in that-negative cases(75% vs 30%, p<0.1). All foci of liver cell dysplasia were positive for p21, 80% of which were strongly positive, and 92% foci of adenomatous hyperplasia were positive for p21. In ECC tissue 67% of cases expressed p21 antigen. All well differentiated HCC cells were stained diffusely but weakly and moderately differentiated HCC cells showed a variety of staining reaction in the morphologic pattern and intensity. However, virtually none of the reaction products was seen in poorly differentiated or undifferentiated areas of HCC.
社団法人日本肝臓学会の論文