:II. Effects of Treatment of Target Cells with Anti-HBs Antibody

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Chang liver cells were coated with purified HBsAg using bisdiazotized benzidine reagents and cytotoxic activity of peripheral blood lymphocyes from patients with chronic hepatitis and healthy subjects against HBsAg-coated Chang cells was studied before and after treatment of HBsAg-coated targed cells with rabbit anti-HBs antibody.Significant T-cell mediated cytotoxicity against HBsAg-coated Chang cells observed in patients with chronic hepatitis was significantly blocked after anti-HBs antibody treatment, indicating that cytotoxic reactions of T-cells were directed against HBsAg on the target cells.Non T-cell cytotoxicity against HBsAg-coated hepatocytes in patients with chronic hepatitis was unchanged before and after treatment with anti-HBs antibody, whereas those of healthy subjects were significantly increased after treatment with anti-HBs antibody. Augumentation of non-T cell cytotoxicity after anti-HBs antibody treatment in healthy subjects was blocked by aggreagated human IgG, indicating that enhanced cytotoxic reactions were mediated by Fc-receptor positive lymhocytes. As a mechanism of the cytotoxicity was seemed to be an Antibody-dependent cell-mediated cytotoxicity. Failure of enhancement of non T-cell cytotoxicity after anti-HBs antibody treatment in patients with chronic hepatitis is considered to be the results of decreased K-cell activity in chronic hepatitis as we reported perviously.
財団法人日本消化器病学会の論文