LYMPHOCYTE CYTOTOXICITY TO COON RAT LIVER CELLS IN CHRONIC LIVER DISEASES

概要

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Lymphocyte cytotoxicity was studied by microcytotoxicity assay in patients with chronic liver diseases. Rat liver cell line, established by Coon, was employed as target liver cells. Specific fluorescence of the membranes of these cells was demonstrated by indirect immunofluorescence using rabbit anti insoluble hepatocyte surface membrane antisera. This result indicates that Coon cells retain liver specificity. Peripheral lymphocyte cytotoxicity to the cells was observed in 26 of 28 patients with chronic active hepatitis. This activity was demonstrated in a fraction of non E-RFC but not in E-RFC from the same patients. On the other hand, cytotoxicity to Chang liver cells was found neither in patients with chronic active hepatitis nor in controls, and lymphocytes from chronic active hepatitis did not show cytotoxic to rat foetal cell line. This suggests that lymphocyte cytotoxicity to Coon cells in patients with chronic active hepatitis may be mediated by liver disease specific lymphocyte reaction.
財団法人日本消化器病学会の論文