Growth control of mature human parenchymal hepatocytes in primary culture: Relationships to collagen synthesis and non-parenchymal liver cells.

概要

論文の詳細を見る
Control of proliferation of human hepatocytes in primary culture was studied. Hepatocytes were isolated by a "two step" method (perfusion followed by shaking) from small specimens of human liver. The hepatocytes could be maintained in monolayer culture for three weeks in serum-free medium, and grew in response to insulin and epidermal growth factor (EGF). L-proline was essential for their DNA synthesis stimulated by these two hormones. Cis-hydroxyproline, a specific inhibitor of collagen synthesis, strongly inhibited DNA synthesis, suggesting that their growth required endogenous synthesis of collagen. In co-cultures of hepatocytes and non-parenchymal liver cells, addition of L-proline, insulin and EGF did not induce DNA synthesis in non-parenchymal liver cells and in fact rather suppressed their DNA synthesis, indicating a reciprocal relation between the growth of these two types of cells. These results should be useful in elucidating the mechanism of liver regeneration, and the pathogenesis of hepatic fibrosis.
社団法人日本肝臓学会の論文