ラット部分切除後の再生肝における胆汁分泌動態の検討

概要

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The present study was performed to investigate sequential changes in bile flow and biliary lipid excretion during liver regeneration after partial hepatectomy (PH) in the rat. Experiments were carried out 1, 3, 7 and 14 days after PH. When sodium taurocholate (TCA) was infused at a rate of 0.5μmol/min/100g b.wt. i.v., bile flow at 1, 3 and 7 days after PH was significantly higher than in controls; especially at the 3 day time point an increase to about 160% of the control level was observed. Biliary excretion of phospholipids and alkaline phosphatase at 3 and 7 days after PH demonstrated paticular augmentation compared with control values. Moreover, the transport maximum of TCA at 1, 3 and 7 days after PH showed a significant increase. Using an isolated liver perfusion system, TCA was administered at rates of 0 to 0.33μmol/min/g liver to groups of non-treated controls and hepatectomized rats 3 days after the operation. Bile flow, biliary phospholipid output and the transport maximum of TCA in the operated group were significantly higher than those observed for control livers, mirroring the results of the in vivo experiments. We therefore conclude that acceleration of bile flow and bile acid excretion into bile after TCA loading of partially hepatectomized rats is dependent on sufficient resupply of phospholipid to the bile canalicular membrane.
社団法人日本肝臓学会の論文