Detection of HIV antigen on acid and heat treated aera from HIV antibodies positive patients.

概要

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Immunoassays for human immunodeficiency virus (HIV) antigens and for antibodies to HIV core and envelope were done on serial sera from 6 patients (3 hemophiliacs and 3 sexual transmitted patients), who had become seropositive on screening by particle agglutination test.Antibodies to HIV envelope by ENVACOR EIA assay were detected in all patients and antibodies to HIV core in 4 of 6 patients. By western blot assay, both gp 41 and gp 120 bands were found in all patients, and the p 24 band were detected in 5 of 6 patients. The results of ENVACOR EIA assay were not exactly compatible with those of western blot assay.The p 24 HIV Ag was positive in only one patient and was negative in 5 patients. But one of 5 negative sera was found to be positive after the treatment of these sera with acid and heat.These results suggest that detection of HIV-Ag after treatment of sera by acid and heat to dissociate antige from immune complex was useful for the disease monitering.
日本臨床免疫学会の論文