The Effect of Heavy Metal Ions on the <I>in Vitro</I> Development of Mouse Embryos: A Comparison of the Developmental Ability between Hams F-10 and α-MEM
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概要
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To elucidate what kind of medium would be desirable for mammalian embryo culture, we evaluated the effect of heavy metal ions on mouse <I>in vitro</I> embryonic development. Pronuclear stage embryos recovered from ICR mice were cultured in Hams F-10 and α-MEM and the developmental ability was compared between them. The major difference of these 2 media is that only Hams F-10 contains heavy metal ions such as Zn<SUP>2+</SUP>, Fe<SUP>2+</SUP> and Cu<SUP>2+</SUP>, and hypoxanthine. When pronuclear stage embryos were cultured in α-MEM, the rates of embryos reaching the 4-cell, blastocyst and hatched blastocyst stages were 96.5%, 75.4% and 66.7%, respectively. These values were significantly (P<0.01) higher than the rates of embryos cultured in Hams F-10; 61.8%, 5.5% and 3.6% respectively. The deletion of CuSO<SUB>4</SUB>, ZnSO<SUB>4</SUB>, FeSO<SUB>4</SUB> and hypoxanthine from Hams F-10 significantly increased the rates of embryos reaching the 4-cell, blastocyst and hatched blastocyst stages to the extent comparable to those in α-MEM. In contrast, the addition of all or one of CuSO<SUB>4</SUB>, ZnSO<SUB>4</SUB>, FeSO<SUB>4</SUB> and/or hypoxanthine to α-MEM significantly decreased the <I>in vitro</I> embryonic development. The strongest inhibition was observed when all of them were added. The developmental ability in α-MEM to which all of them were added was as low as that in Hams F-10. These results suggest that the low developmental ability in Hams F-10 may be mainly due to the deleterious effect of heavy metal ions and hypoxanthine. The toxic effect of heavy metal ions and hypoxanthine might be interpreted as the damage on embryos by an increased generation of oxygen radicals and the medium without constituents which may enhance the production of oxygen radicals seems to be desirable for the culture of mammalian embryos.
- 日本繁殖生物学会の論文
著者
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Mori Takahide
Department Of Gynecology And Gbstetrics Faculty Of Medicine Kyoto University
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GOTO YASUO
Department of Gynecology and Obstetrics, Kyoto University Faculty of Medicine
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Matsumoto Hisashi
Department Of Cardiology Mimihara General Hospital
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Noda Yoichi
Department Of Biotechnology University Of Tokyo
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Matsumoto H
Tohoku Univ. Sendai-shi Jpn
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KISHI Junji
Department of Gynecology and Obstetrics, Kyoto University Faculty of Medicine
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NONOGAKI Takafumi
Department of Gynecology and Obstetrics, Kyoto University Faculty of Medicine
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GOTO Yasuo
Department of Gynecology and Obstetrics
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