Estradiol-induced GnRH Surge in Ovariectomized Goats
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概要
- 論文の詳細を見る
Neurosecretion of hypothalamic GnRH was monitored during the LH surge in ovariecto-mized goats given estradiol using microdialysis sampling. The microdialysis probe with a polycarbon-ate dialysis membrane was stereotaxically implanted in the median eminence, and was perfused with the artificial cerebrospinal fluid at a flow rate of 5μl/min under conscious and unrestrained conditions. The LH surge was induced by means of an intravenous infusion of estradiol (3 μg/h, i.v.) for 16 h and hourly fractions of microdialysis perfusate were collected for GnRH measurement. The perfusate GnRH showed a marked increase to peak levels of 25.3±6.9 μg/ml over basal levels of 2.6±1.1μg/ml at the onset of the LH surge, and then gradually decreased so that the GnRH surge lasted longer than the LH surge. These results suggest that the LH surge is induced by a surge release of GnRH from the hypothalamus but ends despite a continued supply of GnRH.
- 日本繁殖生物学会の論文
著者
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Tanaka Tomomi
Laboratory Of Veterinary Reproduction Tokyo University Of Agriculture And Technology
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Mori Yuji
Laboratory Of Veterinary Ethology Animal Resource Science The University Of Tokyo
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YAMAGUCHI Mitsuo
Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
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MANABE Yasuhiro
Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
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MANABE Yasuhiro
Laboratory of Veterinary Reproduction, Tokyo University of Agriculture and Technology
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YAMAGUCHI Mitsuo
Laboratory of Veterinary Reproduction, Tokyo University of Agriculture and Technology
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