Microdialysis Measurement of <I>In Vivo</I> Gonadotropin-Releasing Hormone Secretion in the Goat
スポンサーリンク
概要
- 論文の詳細を見る
The microdialysis technique was applied to examine the pattern of gonadotropin-releasing hormone (GnRH) secretion in the mediobasal hypothalamus of the goat using a probe with a polycarbonate membrane. An <I>in vitro</I> recovery test showed that changes in the GnRH concentration in the microdialysis perfusate were highly correlated (r=0.996) with those in the external medium, and the relative recovery rate was 2.8% at a flow rate of 5μl/min. Then an <I>in vivo</I> study was carried out in ovariectomized goats which had been implanted stereotaxically with a microdialysis probe in the median eminence of the hypothalamus. Artificial cerebrospinal fluid was perfused and every 10 min fractions were collected under conscious and unrestrained conditions. Patterns of perfusate GnRH were pulsatile and closely associated with LH pulses in the peripheral circulation suggesting that the microdialysis technique can be successfully adopted for examining the neurosecretory activity of GnRH neurons in the conscious goat.
- 日本繁殖生物学会の論文
著者
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Tanaka Tomomi
Laboratory Of Veterinary Reproduction Tokyo University Of Agriculture And Technology
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HOSHINO Kunio
Laboratory of Veterinary Reproduction, Tokyo University of Agriculture and Technology Fuchu
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Mori Yuji
Laboratory Of Veterinary Ethology Animal Resource Science The University Of Tokyo
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Yamaguchi M
Tokyo Univ. Agriculture And Technology Tokyo Jpn
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YAMAGUCHI Mitsuo
Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
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MANABE Yasuhiro
Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
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HOSHINO Kunio
Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
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