喉頭病変の二次元電気泳動法による分析の試み

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A laryngeal white lesion (a LWL) often occurs in the vocal cords. It is often difficult to differenciate a malignant LWL from a non-malignant one under surgical microscope. The treatment is determined on the basis of a histpathological diagnosis from a biopsy specimen. But the reports sometimes differ and one histpathologist diagnoses a LWL as a non-malignant, such as epithelial hyperplasia or dysplasia, and the other diagnoses it as a malignant as cancer. Additional criterion is necessary to determine the true nature of a LWL.Protein is the most abundant organic component of the tissue, and its changes in component will be readily detected by biochemical procedures. The aim of the present report is to detect the changes of both the type and amount of protein so as to serve as a criterion to determine the malignancy of LWL.It is supposed that squamous epithlium contains certain characteristic proteins and a LWL contains some different ones. A comparison between the normally occuring proteins and the different ones has been used to detect dysplasia in tissue. OFarrells two-dimensional electrophoresis was used as it has a high resolution based on charges and molecular weight (MW) of proteins.Laryngeal polyp was used as a control. A leucoplakia and a laryngeal or hypopharyngeal cancer (squamous cell carcinoma) showed specific protein patterns. A laryngeal polyp showed a T-type pattern composed of four protein spots in the pH between 4.6 and 5.0 and in the MW between 40.000 and 50.000. A leucoplakia showed a broad protein pattern in the pH between 4.8 and 5.2, and in the MW between 45.000 and 60.000, which indicates a remarkableincrease in keratin. Laryngeal cancer showed a narrow I-type pattern in the MW between 40.000 and 50.000.
社団法人日本耳鼻咽喉科学会の論文

喉頭病変の二次元電気泳動法による分析の試み

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