ウシプロラクチンに関する免疫化学的研究

概要

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The antisera to bovine prolactin preparation (NIH-P-BI) were obtained by intradermal injections of prolaction with FREUND'S complete adjuvant to rabbit's foot pads and subcutaneously. The characteristics of antisera were determined by precipitin ring test, OUCHTERLONYdiffusion test and anti-hormone test.1) Bovine and ovine prolactin preparations showed complete immunological cross reactionsagainst bovine prolactin antisera. There were no detectable precipitin lines with serumproteins and other pituitary hormones except bovine and ovine growth hormone preparationsat high concentration in gel diffusion studies. The immunological cross reactions with bovineand ovine growth hormone preparations were originated from the contaminating prolactin inthe growth hormone preparations and not from growth hormone itself.2) The antisera showed complete immunological cross reactions with bovine, ovine andgoat's pituitary extracts as in the case of purified bovine and ovine prolactin. On the otherhand, the pituitary extracts from horse, pig, rabbit, rat and chicken did not show anyremarkable cross reactions against bovine prolactin antisera.3) The pigeon's local crop-sac reaction with bovine prolactin was completely inhibited byantisera to bovine prolactin.
社団法人日本畜産学会の論文