セルラーゼによる粗飼料の乾物消化率推定法に関する研究-3-Two step法の乾物消化率推定への応用

概要

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A two step method for estimation of dry matter digestibility (DMD) of roughage was described. In the case of 5% Na2SO3 pretreatment, it is necessary to control the cellulase concentration and/or hydrolysis time in relation to lignin+silica contents of dry matter, i e., lingin+silica contents should be divided as follows; beyond 14%, about 10%, between 6 to 9%, below 5% and be applied to the reaction condition of 5 to 6-hour hydrolysis at 0.05% cellulase, 4-hour at 0.2%, 6-hour at 0.2%, 8-hour at 0.2% respectively. By the application of these experimental condition, we obtained Two Step DMD which agreed very well with in vivo ones with high reproducibility. In the case of hot water pretreatment, there was most high correlation, r=0.934 (p<0.01) between in vivo and Two Step DMD by 8-hour hydrolysis at 0.2% cellulase.
社団法人日本畜産学会の論文