セルラーゼによる粗飼料の乾物消化率推定法に関する研究-1-セルラーゼの性質および細胞膜構造炭水化物の分解反応

概要

論文の詳細を見る
It is the purpose of this investigation to establish the technical system of predicting digestibility of various hays in the laboratory scale. As the introduction servey for this purpose, the digestion trials of the cell wall carbohydrates by cellulase were carried out.Cellulase used in this experiments was Cellulase Onozuka P 1, 500 and substrates were isolated cell wall materials from several grass hays by neutral detergent solution. Cellulase solution was prepared in acetate baffer solution. 300mg of isolated cell wall materials took place in the presence of 45ml of cellulase solution in 50ml polyethylene tube placed in a shaking apparatus at 40°C.The results obtained were as follows:1. The optimum pH of the digestion was around 4.0 to 5.0.2. In the region of inaccessibility of the cell wall carbohydrates, the reaction may be written as follow. x=ctn(x: rate of digestion %, t: reaction time hr., c, n: constant)3. High correlation was observed between in vivo cell wall digestibility and that of cellulase hydrolysis in the case of 4, 8, 12 hour incubation (r=0.997, 0.999, 0.997p<0.01, respectively)
社団法人日本畜産学会の論文