Macelignan Inhibits Melanosome Transfer Mediated by Protease-Activated Receptor-2 in Keratinocytes
スポンサーリンク
概要
- 論文の詳細を見る
Skin pigmentation is the result of melanosome transfer from melanocytes to keratinocytes. Protease-activated receptor-2 (PAR-2) is a key mediator of melanosome transfer, which occurs as the melanocyte extends its dendrite toward surrounding keratinocytes that take up melanosomes by phagocytosis. We investigated the effects of macelignan isolated from Myristica fragrans HOUTT. (nutmeg) on melanosome transfer and the regulation of PAR-2 in human keratinocytes (HaCaT). HaCaT cells stimulated by the PAR-2-activating peptide Ser-Leu-Ile-Gly-Arg-Leu-NH2 (SLIGRL) were treated with macelignan; PAR-2 expression was then determined by reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunocytochemistry. We evaluated the effects of macelignan on calcium mobilization and keratinocyte phagocytosis. In addition, B16F10 melanoma cells and keratinocytes were co-cultured to assess the effects of macelignan on prostaglandin E2 (PGE2) secretion and subsequent dendrite formation. Macelignan decreased HaCaT PAR-2 mRNA and protein levels in a dose-dependent manner. Furthermore, macelignan markedly reduced intracellular calcium mobilization and significantly downregulated keratinocyte phagocytosis, as shown by decreased ingestion of Escherichia coli bioparticles and fluorescent microspheres. In co-culture experiments, macelignan reduced keratinocyte PGE2 secretion, thereby preventing dendrite formation in B16F10 melanoma cells compared with SLIGRL-treated controls. Macelignan inhibits melanosome transfer by downregulating PAR-2, thereby reducing keratinocyte phagocytosis and PGE2 secretion, which in turn inhibits dendrite formation in B16F10 melanoma cells. Taken together, our findings suggest that macelignan could be used as a natural depigmenting agent to ameliorate hyperpigmentation.
著者
-
HWANG Jae-Kwan
Department of Biotechnology, Yonsei University
-
Hwang Jae-kwan
Department Of Biomaterials Science And Engineering Yonsei University
-
CHOI Eun-Jung
Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea
-
Choi Eun-jung
Department Of Microbiology And Immunology College Of Medicine The Catholic University Of Korea
-
Kang Young-Gyu
Department of Biomaterials Science and Engineering, Yonsei University
-
Kim Jaekyung
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University
-
Choi Eun-Jung
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University
関連論文
- Effects of Panduratin A Isolated from Kaempferia pandurata ROXB : on the Expression of Matrix Metalloproteinase-9 by Porphyromonas gingivalis Supernatant-Induced KB Cells(Biopharmacy)
- Depigmentation of Melanocytes by Isopanduratin A and 4-Hydroxypanduratin A Isolated from Kaempferia pandurata ROXB(Biopharmacy)
- Xanthorrhizol, a Natural Sesquiterpenoid, Induces Apoptosis and Growth Arrest in HCT116 Human Colon Cancer Cells
- Estrogenic Activity of Xanthorrhizol Isolated from Curcuma xanthorrhiza ROXB.
- The effect of 4-hydroxypanduratin A on the mitogen-activated protein kinase-dependent activation of matrix metalloproteinase-1 expression in human skin fibroblasts
- Macelignan Inhibits Melanosome Transfer Mediated by Protease-Activated Receptor-2 in Keratinocytes
- Association of HLA alleles with non-Hodgkin's lymphoma in Korean population
- Protective Effects of Macelignan on Cisplatin-Induced Hepatotoxicity Is Associated with JNK Activation(Biopharmacy)
- Ginsenoside 20(S)-Protopanaxatriol (PPT) Activates Peroxisome Proliferator-Activated Receptor γ (PPARγ) in 3T3-L1 Adipocytes(Pharmacology)
- Suppressive effect of ethanolic Kaempferia pandurata Roxb. extract on matrix metalloproteinase-2 expression in Porphyromonas gingivalis-treated human gingival fibroblasts in vitro
- Inhibitory Effect of Panduratin A on c-Jun N-Terminal Kinase and Activator Protein-1 Signaling Involved in Porphyromonas gingivalis Supernatant-Stimulated Matrix Metalloproteinase-9 Expression in Human Oral Epidermoid Cells
- Antioxidant Properties of Neohesperidin Dihydrochalcone : Inhibition of Hypochlorous Acid-Induced DNA Strand Breakage, Protein Degradation, and Cell Death(Medicinal Chemistry)
- Effects of macelignan isolated from Myristica fragrans Houtt. on UVB-induced matrix metalloproteinase-9 and cyclooxygenase-2 in HaCaT cells
- Inhibitory Effects of Macelignan Isolated from Myristica fragrans HOUTT. on Melanin Biosynthesis(Miscellaneous)
- Immunostimulating Activity of Crude Polysaccharide Extract Isolated from Curcuma xanthorrhiza Roxb
- Immunostimulating Activity of Crude Polysaccharide Extract Isolated from Curcuma xanthorrhiza Roxb.
- Effects of (+)-Catechin on the Function of Osteoblastic Cells
- meso-Dihydroguaiaretic Acid Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated Protein Kinase in Human HepG2 Cells
- In Vitro Antibacterial Activity of Panduratin A against Enterococci Clinical Isolates
- Effects of Macelignan Isolated from Myristica fragrans (Nutmeg) on Expression of Matrix Metalloproteinase-1 and Type I Procollagen in UVB-Irradiated Human Skin Fibroblasts
- Activity of panduratin A isolated from Kaempferia pandurata Roxb. against multi-species oral biofilms in vitro