Computational Analysis of the Effects of Antineoplastic Agents on Axonal Transport
スポンサーリンク
概要
- 論文の詳細を見る
Axonal transport plays a crucial role in neuronal morphogenesis, survival, and function. Despite its importance, however, the molecular mechanisms of axonal transport remain mostly unknown because a simple and quantitative assay system for axonal transport has been lacking. In order to better characterize the molecular mechanisms involved in axonal transport, we here developed a computer-assisted monitoring system. Using lipophilic fluorochrome chloromethylbenzamido dialkylcarbocyanine (CM-DiI) as a labeling dye, we have successfully labeled membranous organelles in cultured chick dorsal root ganglia neurons. We confirmed that sodium azide, an ATPase inhibitor, and nocodazole, a microtubule-destabilizing agent, markedly suppressed anterograde and retrograde axonal transport of CM-DiI–labeled particles. We further tested the effects of several anti-neoplastic drugs on axonal transport. Paclitaxel, vincristine, cisplatin, and oxaliplatin, all of which are known to be neurotoxic and to cause neurological symptoms, suppressed anterograde and retrograde axonal transport. Another series of anti-neoplastic drugs, including methotrexate and 5-fluorouracil, did not affect the axonal transport. This is the first report of an automated monitoring system for axonal transport. This system will be useful for toxicity assays, characterizing axonal transport, or screening drugs that may modify neuronal functions.
著者
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ICHIKAWA YASUSHI
Department of Surgery-2, Yokohama City University, School of Medicine
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Gotoh Toshiyuki
Graduate School Of Environment And Information Sciences Yokohama National University
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YAMASHITA Naoya
Department of Pediatrics, Keio University School of Medicine
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GOSHIMA Yoshio
Department of Pharmacology, Yokohama City University School of Medicine
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Ichikawa Yasushi
Department Of Clinical Oncology Yokohama City University Graduate School Of Medicine
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Usui Hiroshi
Department Of Molecular Pharmacology And Neurobiology Yokohama City University Graduate School Of Me
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Goshima Yoshio
Department Of Molecular Pharmacology And Neurobiology Yokohama City University Graduate School Of Me
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SHIOZAWA Takahito
Graduate School of Environment and Information Sciences, Yokohama National University
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HIDA Tomonobu
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of M
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KURAOKA Satoshi
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of M
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TAKESHITA Sayumi
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of M
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KAMIYA Yoshinori
Department of Anesthesiology, Yokohama City University Graduate School of Medicine
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GOTOH Takahisa
Department of Anesthesiology, Yokohama City University Graduate School of Medicine
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Hida Tomonobu
Department Of Anesthesiology And Critical Care Medicine Yokohama City University Graduate School Of
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Yamashita Naoya
Department Of Molecular Neuropharmacology Yokohama City University Graduate School Of Medicine
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Goshima Yoshio
Department Of Molecular Neuropharmacology Yokohama City University Graduate School Of Medicine
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Gotoh Takahisa
Department Of Anesthesiology And Critical Care Medicine Yokohama City University Graduate School Of
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Kuraoka Satoshi
Department Of Molecular Pharmacology And Neurobiology Yokohama City University Graduate School Of Me
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Takeshita Sayumi
Department Of Molecular Pharmacology And Neurobiology Yokohama City University Graduate School Of Me
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Kamiya Yoshinori
Department Of Anesthesiology And Critical Care Medicine Yokohama City University Graduate School Of
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Shiozawa Takahito
Graduate School Of Environment And Information Sciences Yokohama National University
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Kuraoka Satoshi
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Japan
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Shiozawa Takahito
Graduate School of Environment and Information Sciences, Yokohama National University, Japan
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Yamashita Naoya
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Japan
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Yamashita Naoya
Department of Electrical Engineering and Information Systems, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Gotoh Takahisa
Department of Anesthesiology, Yokohama City University Graduate School of Medicine, Japan
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Goshima Yoshio
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Japan
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Ichikawa Yasushi
Department of Clinical Oncology, Yokohama City University Graduate School of Medicine, Japan
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Takeshita Sayumi
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Japan
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Kamiya Yoshinori
Department of Anesthesiology, Yokohama City University Graduate School of Medicine, Japan
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Gotoh Toshiyuki
Graduate School of Environment and Information Sciences, Yokohama National University, Japan
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