Preparation and catalytic evaluation of cytochrome c immobilized on mesoporous silica materials
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概要
- 論文の詳細を見る
Cytochrome c (cyt. c, molecular size of 2.5 nm × 2.5 nm × 3.7 nm) was immobilized on six types of mesoporous silicates (MPS), having pore sizes from 2.8 to 15.0 nm, and their catalytic activities were evaluated by oxidation of thioanisol with H2O2. Among the silica materials, cyt. c immobilized on MPS pore size of 3.4 nm) showed the best catalytic activity, and its relative activity was improved 1.7 times over that of cyt. c solution. Enhancement of catalytic activity was obtained by immobilization on MPS having a similar size (mesopore) to the molecular size of cyt. c. Activity stability of cyt. c was also evaluated under the various conditions that caused protein inactivation, like HCl, urea, methanol, and guanidine. As results, stability was improved by the immobilization on silica material having a larger pore size (15.0 nm), offered an environment for encapsulating entire cyt. c molecules, inhibiting cyt. c from unfolding and irreversible decomposition.
- 公益社団法人 日本セラミックス協会の論文
著者
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TANEMURA Masaki
Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology
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Saito Takao
National Institute of Advanced Industrial Science and Technology (AIST)
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Kato Katsuya
National Industrial Research Insitute Of Nagoya
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Tanemura Masaki
Department Of Environmental Technology Graduate School Of Engineering Nagoya Institute Of Technology
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Suzuki Makoto
Department Of Cardiology The Second Tokyo National Hospital
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Suzuki Makoto
Department Of Cardiology Kameda Medical Center
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Suzuki Makoto
Department Of Cardiology Ehime Central Prefectual Hospital
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Tanemura Masaki
Department of Environmental Technology & Urban Planning, Nagoya Institute of Technology, Gokisho-cho, Showa-ku, Nagoya 466-8555, Japan
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SUZUKI Makoto
Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology
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