Development of in Situ Hybridization for the Detection of Mycoplasma hyorhinis in Formalin-Fixed Paraffin-Embedded Tissues from Naturally Infected Pigs with Polyserositis

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概要

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• The aim of this study was to develop in situ hybridization for the detection of Mycoplasma hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. M. hyorhinis was isolated from the spleen (2 pigs) and pericardium (1 pig). M. hyorhinis DNA was detected 16 out of 20 pigs with polyserositis. In situ hybridization produced a distinct positive signal for the M. hyorhinis p37 gene in inflammatory cells in the polyserositis. In situ hybridization developed in the present study present diagnostic tools capable of detection of M. hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from naturally infected pigs.

著者

• Kim Chung

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• Oh Yeonsu

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• Ha Yooncheol

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• CHAE Chanhee

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• Chae Chanhee

  Department Of Veterinary Pathology College Of Veterinary And School Of Agricultural Biotechnology Se

• Kim Chung

  Department Of Chemistry Indiana University

• KIM Bongtae

  Department of Veterinary Pathology, College of Veterinary Medicine and School of Agicultural Biotech

• Kim Bongtae

  Department Of Physiology & Immunology School Of Medicine Konkuk University

• LEE Kichan

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• HAN Kiwon

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• KIM Duyeol

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• KANG Ikjae

  Dong Bang Co., Ltd.

• LEE Jeehoon

  Dong Bang Co., Ltd.

• KIM Duyeol

  Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

• HA Yooncheol

  Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

• LEE Kichan

  Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

• HAN Kiwon

  Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

• KIM Bongtae

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

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