Comparative Study of In situ Hybridization and Immunohistochemistry for the Detection of Porcine Circovirus 2 in Formalin-Fixed, Paraffin-Embedded Tissues
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概要
- 論文の詳細を見る
In situ hybridization and immunohistochemistry with different types of antibody (monoclonal vs. polyclonal, natural vs. synthetic) was compared to detect porcine circovirus 2 (PCV2) in formalin-fixed, paraffin-embedded tissues from pigs with experimentally and naturally occurring postweaning multisystemic wasting syndrome. PCV2 DNA and antigen was detected in tissues from both experimentally and naturally infected pigs by in situ hybridization and immunohistochemistry, respectively. Statistical evaluation revealed that more PCV2 positive signals were significantly detected in both experimentally and naturally infected pigs by in situ hybridization compared with immunohistochemistry (P<0.05). The results of this study demonstrated that in situ hybridization proved more sensitive than immunohistochemistry for the detection of PCV2 in formalin-fixed, paraffin-embedded lymph node tissues.
- 社団法人 日本獣医学会の論文
著者
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Ha Yooncheol
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University
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KIM Sung-Hoon
College of Oriental Medicine, Kyunghee University
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Chae Chanhee
Department Of Veterinary Pathology College Of Veterinary And School Of Agricultural Biotechnology Se
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Kim Junghyun
Department Of Veterinary Pathology College Of Veterinary Medicine And School Of Agricultural Biotech
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Lee Yong-hoon
Department Of Mathematics Pusan National University
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LEE Kichan
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University
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HAN Kiwon
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University
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KIM Duyeol
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University
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CHAE Sungwon
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotec
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LEE Ju-Ho
College of Oriental Medicine, Kyunghee University
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HWANG Kyu-Kye
Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Jeju National University
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Kim Sung-Hoon
College of Oriental Medicine, Kyung Hee University
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KIM Duyeol
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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CHAE Sungwon
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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HA Yooncheol
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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LEE Kichan
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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HAN Kiwon
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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KIM Junghyun
Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine
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LEE Yong-Hoon
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
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