Antimicrobial Resistance and Genetic Characteristics of Salmonella Typhimurium Isolated from Horses in Hokkaido, Japan

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概要

• 論文の詳細を見る

• In this study, we examined the antimicrobial susceptibility of 16 Salmonella Typhimurium isolates obtained from horses, and applied several genetic methods, namely polymerase chain reaction (PCR) for detecting class 1 integrons, multiplex PCR for detecting multidrug resistant S. Typhimurium definitive phage type 104 (MR-DT104), and fluorescent amplified-fragment length polymorphism (FAFLP). Seven isolates with an ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline (ACSSuT) type resistance pattern, harbored two class 1 integrons with sizes of 1.2 and 1.0 kb, and were identified as DT104 by bacteriophage typing. These isolates also showed a typical MR-DT104 amplification pattern, which was positive for flost, spvC, invA and int, in multiplex PCR. In the FAFLP analysis, the equine DT104 isolates and the previously reported ACSSuT-type resistant bovine isolates, which were also isolated in Hokkaido were included in the same genetic cluster. Our results retrospectively indicate that MR-DT104 infection has existed in horses in Japan at least since 1996, and it was suggested that there is a highly epidemiological relationship between the equine MR-DT104 isolates and certain multidrug resistant bovine isolates in the same area.

• 社団法人　日本獣医学会の論文

著者

• TOZAKI Teruaki

  Department of Molecular Genetics, Laboratory of Racing Chemistry

• Anzai Toru

  日本中央競馬会競走馬理化学研究所

• Hobo Seiji

  Microbiology Division Epizootic Research Center Equine Research Institute Japan Racing Association

• Uchida Ikuo

  Hokkaido Research Station National Institute Of Animal Health:united Graduate School Of Veterinary S

• Izumiya Hidemasa

  Department Of Bacteriology National Institute Of Infectious Disease

• Watanabe Haruo

  Department Of Bacteriology I National Institute Of Infectious Diseases

• NIWA Hidekazu

  Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Associatio

• ANZAI Toru

  Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Associatio

• MORITA-ISHIHARA Tomoko

  Department of Bacteriology, National Institute of Infectious Diseases

• Anzai Toru

  Equine Research Institute Epizootic Research Station

• Uchida Ikuo

  Hokkaido Res. Station National Inst. Of Animal Health

• Izumiya Hidemasa

  Department of Bacteriology I, National Institute of Infectious Diseases

• ANZAI Toru

  Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association

• NIWA Hidekazu

  Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association

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