Binding Energy of Tea Catechin/Caffeine Complexes in Water Evaluated by Titration Experiments with ^1H-NMR
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概要
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Evaluating the binding energy of a catechin/caffeine complex in water is important in order to elucidate the ability for molecular recognition of tea catechins. The results of this study revealed that the stoichiometric ratio of the complexation between tea chatechins (EGCg, ECg, EGC, and EC) and caffeine was 1:1 at least up to a concentration of 5.0 mM. The free energy (−ΔG) values for binding in water at 301 K were evaluated to be 2.7, 2.6, 2.2, and 2.0 kcal/mol for EGCg, ECg, EGC, and EC, respectively, by the titration method with 1H-NMR. An investigation of the 1H-NMR chemical shift change and NOESY spectra in the catechin/caffeine solutions showed the participation of the A-rings of the catechins in complexation, as well as that of the galloyl groups or B-rings.
- 2004-12-23
著者
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KOHATA KATSUNORI
National Institute of Vegetables, Ornamental Plants and Tea
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Ujihara Tomomi
National Institute of Vegetable and Tea Science
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HAYASHI Nobuyuki
National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Orga
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Kohata Katsunori
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
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Ujihara Tomomi
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
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Hayashi Nobuyuki
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
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Hayashi Nobuyuki
National Agriculture And Food Res. Organization (naro) Shizuoka Jpn
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KOHATA Katsunori
National Institute of Vegetable and Tea Science
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