High Performance Liquid Chromatographic Determination of Pheophorbide-a and Its Related Chlorophyll Derivatives in Tea Leaves.
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概要
- 論文の詳細を見る
A simple method for the extraction of tea samples and conditions of HPLC analysis of pheophorbide-a (PB-a) and its related chlorophyll derivatives was developed. Tea samples were extracted with 85% acetone (v/v) with this solution injected directly into the HPLC column. The modified HPLC procedure developed included a gradient solvent system in which solvent A (95% ethanol (v/v) containing 0.005 M sodium chloride) and solvent B (80% ethanol (v/v) containing 0.005 M sodium chloride) were the mobile phase. PB-a, its derivatives and their C-10 epimers could be clearly separated and determined within 35 min. This analytical method could be routinely used to determine low levels of PB-a content (<10 mg/100g) and its related individual chlorophyll derivatives in green teas. Hence, it is applicable to the safety and quality control of green teas.
- 社団法人 日本食品科学工学会の論文
著者
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Horie Hideki
National Institute of Vegetable and Tea Science
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Hanada Kouichi
Itochu Sugar Co. Ltd.
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KOHATA Katsunori
National Institute of Vegetable and Tea Science
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KOHATA Katsunori
National Research Institute of Vegetable, Ornamental Plants and Tea
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