Isolation and Identification of Sphingomonas sp. That Yields tert-Octylphenol Monoethoxylate under Aerobic Conditions

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概要

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• Topsoil samples were collected from eight golf courses in Yamaguchi Prefecture, Japan, and enrichment cultures were carried out with a basal-salt medium containing 0.2% 4-tert-octylphenol polyethoxylate (OPPEO) as sole carbon source. OPPEO-degrading activity was detected in one of the samples, from which a strain of OPPEO-degrading bacterium was isolated. The isolated bacterium grew on a nutritionally enriched medium (NE medium) containing 0.2% OPPEO as sole carbon source, and accumulated 4-tert-octylphenol diethoxylate (OP2EO) (63%), 4-tert-octylphenol triethoxylate (OP3EO) (14%), and 4-tert-octylphenol monoethoxylate (OP1EO) (2%) after 7 d cultivation under aerobic conditions. The addition of clay mineral (vermiculite) to the medium accelerated the degradation of OP2EO (40%) and OP3EO (4%) to OP1EO (23%). This is the first report about bacteria that can degrade OPPEO to OP1EO under aerobic conditions. The strain was identified as Sphingomonas macrogoltabidus, based on the homology of a 16S rDNA sequence.

• 社団法人 日本農芸化学会の論文
• 2005-07-23

著者

• Tomita Yoshifumi

  Department Of Internal Medicine And Cardiology Nippon Medical School Tama-nagayama Hospital

• Tomita Yoshifumi

  Department Of Environmental Chemistry Kyushu Kyoritsu University

• NISHIO Eriko

  Department of Environmental Chemistry, Kyushu Kyoritsu University

• YOSHIKAWA Hiromichi

  Department of Functional Materials Engineering, Fukuoka Institute of Technology

• WAKAYAMA Manabu

  Department of Applied Biological Chemistry, Meijo University

• TAMURA Hiroto

  Department of Applied Biological Chemistry, Meijo University

• MORITA Shiro

  Department of Environmental Chemistry, Kyushu Kyoritsu University

• Tamura H

  Department Of Environmental Bioscience Faculty Of Agriculture Meijo University

• Morita Shiro

  Department Of Agricultural Chemistry The University Of Tokyo

• Nishio Eriko

  Department Of Environmental Chemistry Kyushu Kyoritsu University

• Tamura Hiroto

  School Of Agriculture Meijo University

• Tomita Yoshifumi

  Department Of Cardiology Tamanagayama Hospital Nippon Medical School

• Wakayama Manabu

  Department Of Applied Biological Chemistry Meijo University

• Tamura Hiroto

  Department Of Applied Biological Chemistry Meijo University

• Morita S

  Department Of Environmental Chemistry Kyushu Kyoritsu University

• Yoshikawa H

  Graduate School Of Engineering Fukuoka Institute Of Technology

• Yoshikawa Hiromichi

  Department Of Life Environmental And Material Science Fit

• Yoshikawa Hiromichi

  Department Of Environmental Chemistry Kyushu Kyoritsu University

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