5-Bromodeoxyuridine Increases Transient Expression of Ectopic Genes in Human Cells
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概要
- 論文の詳細を見る
Ectopic genes transferred to cells are temporally expressed, although this phenomenon has not yet been well characterized. We found that 5-bromodeoxyuridine dramatically increased transient expression of ectopic genes in human cells. This effect was elicited by adding 5-bromodeoxyuridine prior to or after transfection. No promoter specificity was observed. Real time PCR analysis showed an approximately 2-fold increase in mRNA levels. Since 5-bromodeoxyuridine decondenses heterochromatin and changes the nuclear envelope, these changes might affect transcriptional and post-transcriptional events in the gene expression of plasmids.
- 社団法人 日本農芸化学会の論文
著者
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Hossain Mohammad
Kihara Inst. For Biological Res. And Graduate School Of Integrated Sci. Yokohama City Univ.
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Ayusawa Dai
Kihara Institute For Biological Research And Graduate School Of Integrated Science Yokohama City Uni
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Fujii Michihiko
Kihara Inst. For Biological Res. And Graduate School Of Integrated Sci. Yokohama City Univ.
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ENDOH Morio
Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City Un
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MIKI Kensuke
Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City Un
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