Z-Nin, a New High Molecular Weight Protein Required for Reconstitution of the Z-Disk
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概要
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The materials released from myofibrils by Ca2+ -activated factor (CAF) were fractionated byusing a combination of Sepharose 6B and Bio-gel A 50m columns. To investigate the exact constituents of Z-disks on the basis of success or failure of reconstitution of Z-disks, reconstitution of Z-disks by incubating individual fractions with Z-disk-extracted fiber bundles was carried out, and the proteins in each fraction were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. From the electron micrographs of the reconstituted Z-disks, SDS-polyacrylamide gel electrophoretic analysis and amino acid analysis of the isolated proteins from the gels, it was clear that the proteins required for the reconstitution of the Z-disk, in other words, the principal constituents of the Z-disk, were Z-nin, a new high molecular weight protein having a subunit molecular weight of 300, 000-400, 000 dalton mentioned in this report, α-actinin and tropomyosin.
- 社団法人 日本農芸化学会の論文
著者
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SAITO Makoto
Electron Microscopic Laboratory, University of Niigata
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Suzuki Atsushi
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
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NONAMI Yoshinobu
Department of Animal Science, Faculty of Agriculture, University of Niigata
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OKITANI Akihiro
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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