Purification and Some Properties of Debranching Enzyme of Germinating Rice Endosperm
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概要
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A debranching enzymewas extracted from the endospermof germinating rice seeds and purified through three steps, namely cyclohexaamylose-coupled Sepharose 6B, Ultrogel AcA-44 and Bio-Gel P-150 column chromatography. This disc-electrophoretically homogeneous enzyme showed a specific activity of 43 units/mg of protein (30°C) with a pH optimum of 5.5. The isoelectric point was 4.9, unlike that (pI 3.5) of debranching enzyme of ungerminated rice seeds. Our enzyme hydrolyzed pullulan rapidly, and glutinous rice starch and waxy corn starch moderately. The enzyme was also able to act on phytoglycogen and glycogen unlike debranching enzymes originating in some plants.
著者
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Fuwa Hidetsugu
Department Of Food And Nutrition Osaka City University
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IWAKI Keiko
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University
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