Mode of Action of an exo-β-(1→3)-D-Glucanase on the Laminaran from Eisenia bicyclis
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概要
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In the initial stage of hydrolysis, exo-β-(1→3)-D-glucanase from Basidiomycetes sp. QM806 cleaved laminaran from Eisenia bicyclis with a pattern resembling an erdo-hydrolase. Five kinds of intermediate gluco-oligosaccharides were separated by a combination of gel filtration and HPLC. They were shown to be 32-O-β-D-glucosyl-gentiobiose, 32-O-β-D-gentiobiosyl-gentiobiose, 33-O-β-D-glucosyl-gentiotriose, 34-O-β-D-glucosyl-32-O-gentiobiosyl-gentiobiose, and 33-O-β-D-gentiobiosylgentiotriose by enzymic hydrolysis and methylation analysis as well as by 13C NMRspectroscopy. As a result, such kinds of β(1→3)-β-(1→6)-linked oligosaccharides could be accounted for in the initial cleavage, and they were hydrolyzed ultimately to glucose, gentiobiose, and gentiotriose. It suggests that a single (1→3)-linkage on a block of (1→6)-links show some resistance to attack by this enzyme.
- 社団法人 日本農芸化学会の論文
著者
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Usui Taichi
Department Of Agricultural Chemistry Faculty Of Agriculture Shizuoka University
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NANJO Fumio
Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University
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SUZKI Tateo
Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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