Separation and Characterization of the endo-β-(1→3)-D-Glucanase from Rizoctonia solani
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概要
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The endo-β-(1→3)-D-glucanase from extracts of Rhizoctonia solani has been purified to homogeneity by chromatographies on CM Bio-Gel A and Bio-Gel P-60 and finally by affinity chromatography on short-chain pachyman-AH-Sepharose. In the affinity chromatography, the adsorption of the enzyme seemed to be brought into close with the large spacer arm by prior biospecific adsorption on an attached ligand. The isoelectric point was pH 9.8 and the molecular weight was 29, 000. The optimum pH for short-chain pachyman was 5.5. The enzyme was fairly stable at pH 5 to 8.5 and at temperatures below 50°C. The enzyme favors streches of β-D-(1→3)-linkages, and also depolymerize β-(1→3)-β-(1→4) mixed-linked glucan. It has transglycosylase activity.
- 社団法人 日本農芸化学会の論文
著者
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Usui Taichi
Department Of Agricultural Chemistry Faculty Of Agriculture Shizuoka University
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TOTSUKA Atsushi
Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University
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