Lysozyme-mediated p-Nitrophenyl Penta-N-acetyl-β-chitopentaoside Production in Aqueous-dimethylsulfoxide Solvent System, as a Substrate for a Lysozyme Assay
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概要
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PNP-(GlcNAc)5, which is available as a substrate of a lysozyme assay, was efficiently synthesized by transfer of the tetra-N-acetyl-chitotetraosyl residue to the 4-position of PNP-GlcNAc using lysozyme in an aqueous solution containing DMSO in a high concentration. The use of an aqueous DMSO system in this reaction not only ensured a sufficient solubility of PNP-GlcNAc substrate, but also resulted in the high yield of PNP-(GlcNAc)5. PNP-(GlcNAc)5 insolubilized from the reaction mixture was directly obtained in a high state of purity by washing with aqueous methanol without using any column chromatographic separation.
- 社団法人 日本農芸化学会の論文
著者
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Usui Taichi
Department Of Agricultural Chemistry Faculty Of Agriculture Shizuoka University
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Matsui Hidenori
Department Of Appied Biological Chemistry Faculty Of Agriculture Shizuoka University
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MATSUI Hidenori
Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University
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