High Yield Preparation of Lentinus edodes ("Shiitake") Protoplasts with Regeneration Capacity and Mating Type Stability
スポンサーリンク
概要
- 論文の詳細を見る
An efficient method for the liberation and regeneration of L. edodes protoplasts was developed using an enzyme mixture of Cellulase Onozuka RS and chitinase, pH 4.6. Homogeneous young mycelia as the protoplast material were quickly cultured in a liquid medium with wood components after inoculation of cut fragments of hyphae. The preparation of more than 6 × 107 protoplasts/ml/4hr and regeneration of more than 15% were attained. The dikaryons formed through the mating of monokaryotic strains of protoplast origin could develop complete fruit bodies on small scale sawdust cultivation (30g medium) in 50-60 days. After protoplasting or fusion treatment with polyethyleneglycol, the mating phenotypes were sufficiently well preserved.
- 社団法人 日本農芸化学会の論文
著者
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Yanagi Sonoe
National Food Research Institute
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KAWASUMI Toshiyuki
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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Kawasumi Toshiyuki
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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OHBA Kazuhiro
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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KIUCHI Nobuyuki
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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FUTATSUGI Yasuji
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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