大腸菌ワクチン投与後のホルスタイン牛初乳中免疫成分とADA活性

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乾乳期の乳牛に大腸菌ワクチン製剤を投与し，初乳中リンパ球数，リンパ球サブセット，免疫グロブリン濃度およびアデノシンディアミナーゼ（ADA）活性を調べた．試験には，臨床的に健康で乳房炎や血乳のなかったホルスタイン種経産牛を，投与群（V群）５頭とコントロール群（C群）５頭の２群に分けて供試した．V群の初乳中白血球はC群と比べて，リンパ球，CD4陽性T細胞数，CD8陽性T細胞数，γδT細胞数，IgM陽性細胞数は差がなかった．V群のADA活性値は7.5±1.9IU/ℓで，C群の12.2±2.9IU/ℓに比べて有意な高値を示した．CD4陽性T細胞数とADA活性値の間に相関性が認められた．V群の初乳中免疫グロブリン濃度はC群と比べて，IgG1濃度とIgA濃度において有意な高値を示し，IgG1濃度とADA活性値の間に相関性が認められた．以上のことから，大腸菌ワクチン投与は初乳において液性免疫増強作用のあること，また，初乳中ADA活性は初乳の液性免疫状態を知る指標になり得ることが示唆された．We examined the number of lymphocytes, lymphocyte subsets, the concentration of immunoglobulins and adenosine deaminase （ADA） activity in colostrum after administration of E. coli vaccine to dairy cows during the dry period. Healthy postparturient Holstein cows, which were negative for both mastitis and bloody milk, were divided into two groups; five were vaccinated （V group） andthe other five were untreated as controls（ C group）. The number of lymphocytes, CD4-positive T cells, CD8-positive T cells, γδT cells and IgM-positive cells was not significantly different between the V and C groups. The mean ADA activity was 17.5 ±1.9 IU/ℓ in the V group and 12.2±2.9 IU/ℓ in the C group, significantly higher in the V group. A significant correlation was observed between the number of CD4-positive T cells and the ADA activity.IgG1 and IgA concentrations were significantly higher in the V group than in the C group. A significant correlation was observed between the concentration of IgG1 and the ADA activity.These results suggest that E. coli vaccine results in reinforcement of humoral immunity in colostrum. In addition, it was suggested that ADA activities in colostrum might be a good indicator of the status of humoral immunity in colostrum.
2011-06-30

大腸菌ワクチン投与後のホルスタイン牛初乳中免疫成分とADA活性

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