DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma
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概要
- 論文の詳細を見る
DOCK2; a member of the CDM protein family, regulates cell motility and cytokine production through the activation of Rac in mammalian hematopoietic cells and plays a pivotal role in the modulation of the immune system. Here we demonstrated the alternative function of DOCK2 in hematopoietic tumor cells, especially in terms of its association with the tumor progression. Immunostaining for DOCK2 in 20 cases of human B cell lymphoma tissue specimens including diffuse large B cell lymphoma and follicular lymphoma revealed the prominent expression of DOCK2 in all of the lymphoma cells. DOCK2-knockdown (KD) of the B cell lymphoma cell lines, Ramos and Raji, using the lentiviral shRNA system presented decreased cell proliferation compared to the control cells. Furthermore, the tumor formation of DOCK2-KD Ramos cell in nude mice was significantly abrogated. Western blotting analysis and pull-down assay using GST-PAK-RBD kimeric protein suggested the presence of DOCK2-Rac-ERK pathway regulating the cell proliferation of these lymphoma cells. This is the first report to clarify the prominent role of DOCK2 in hematopoietic malignancy.
- 2010-04-23
著者
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Endo Tomoyuki
Stem Cell Transplantation Center Hokkaido University Hospital
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Takeuchi Jin
Department Of Hematology And Rheumatology Nihon University School Of Medicine
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Onozawa Masahiro
Stem Cell Transplantation Center Hokkaido University Hospital
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Nishio Mitsufumi
Stem Cell Transplantation Center Hokkaido University Hospital
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Kondo Takeshi
Stem Cell Transplantation Center Hokkaido University Hospital
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Kawamura Takahito
Department Of Medicine Ii Hokkaido University Graduate School Of Medicine
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Kondo Takeshi
北海道大学 医学研究科消化器内科学
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Onozawa Masahiro
北海道大学 医学研究科消化器内科学
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Obara Masato
Department Of Internal Medicine Ii Hokkaido University Graduate School Of Medicine
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Kondo Takeshi
Department Of Medicine Ii Hokkaido University Graduate School Of Medicine
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Tanaka Junji
Japan Society For Hematopoietic Cell Transplantation Adult Data Management Committee
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Kondo Takeshi
Department Of Pathology Laboratory Of Cancer Research Hokkaido University Graduate School Of Medicine
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Tanaka Shinya
Department Of Pathology Laboratory Of Cancer Research Hokkaido University Graduate School Of Medicine
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