A14 超音波を用いた蛋白質のアミロイド線維形成反応の促進

概要

論文の詳細を見る
Amyloid fibrils are highly ordered assemblies of misfolded proteins associated with over 30 degenerative diseases including Alzheimer's diseases or type 2 diabetes. Fibrils form through nucleation and growth. This is similar to crystallization. Fibrillation takes a long time because of the high free-energy barrier to nucleation. Understanding the mechanisms of fibrillation is useful to treat amyloid associated diseases. However, studies have been limited by a long lag phase and low reproducibility of fibrillation. Ultrasonication can generate fibrils in a short time and a microplate reader can detect fibrils in many samples simultaneously. Here, we propose the amyloid-screening system with a combined use of ultrasonicator and microplate reader. Further, we show the correlation of fibrillation with ultrasonic amplitude. The results indicate that fibrillation is a physical reaction that is largely limited by high free-energy barrier, which can be effectively reduced by ultrasonication. This approach will contribute to the understanding of the amyloidogenicity of various proteins and to the creation of therapeutic strategies against a wide range of degenerative diseases.
日本ソノケミストリー学会の論文
2012-11-09