Motility behavior of rpoS-deficient Escherichia coil analyzed by individual cell tracking(BIOCHEMICAL ENGINEERING)
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概要
- 論文の詳細を見る
Motility is one of the most extensively studied cellular events conducted by bacteria, including Escherichia coil. A motility agar plate assay showed that deletion of the rpoS gene enhanced the apparent motility of the E. coll BW25113 strain, which inherently had negligible motility compared to wild-type E. coli strains, such as MG1655, with no effect on cell growth. This enhancement of motility was accompanied by drastic up-regulation of genes involved in the formation and rotation of flagella. Furthermore, an individual cell motility assay showed that the population of ΔrpoS cells had bimodal motility character, and that a minority of this population exhibited a much higher motility rate. These results support a view that a minority population contributes to increasing in apparent motility of the whole population of ΔrpoS cells.
- 公益社団法人日本生物工学会の論文
- 2012-12-00
著者
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Taya Masahito
Division Of Chemical Engineering Department Of Materials Engineering Science Graduate School Of Engi
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Ojima Yoshihiro
Division Of Chemical Engineering Department Of Materials Engineering Science Graduate School Of Engi
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Nguyen Minh
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University
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Taya Masahito
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University
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Hakamada Kazumi
Division of Bioengineering, Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
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Nishinoue Yosuke
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University
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Miyake Jun
Division of Bioengineering, Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
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