薄層クロマトグラフィーデンシトメトリーの生薬分析への応用(第1報) : 南天および南天製剤中のアルカロイドの定量

概要

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A simple and rapid determination of O-methyldomesticine (I) in Nandina fruits and its syrup preparations has been deviced using thin-layer chromatography-densitometry(TLC-DM) system. Soxhlet extraction of Nandina fruits with ammonia-methanol (1:10) for 4 hours brought good results. Development of the extracts spotted on Kieselgel G (250μ thick) was carried out with the following solvents: benzene-ethyl acetate-diethylamine (7:2:1), and methanol. In order to determine (I), UV reflective absorption at 310 nm were measured by Densitometer CS-900 (Shimadzu Co.) with zig-zag-scanning method. As the lightabsorption was slightly differed from each other, calibration curve was made up for each plate. The alkaloid contents in three different Nandina samples were 0.50, 0.47, and 0.45% by titrimetric method, while by TLC-DM, they were 0.45, 0.41 and 0.35%, respectively. Recoveries from fortified syrups were nearly 90%. In practice, however, only 10% of (I) calculated from the content in the starting material was found in the commercial syrup preparations. This is due to the poor extracting efficiency of 20% ethanol which is used for the preparation of Nandina extract.
日本生薬学会の論文
1975-06-20