柴胡エキス中のSaikosaponinの定量(第1報) : Saikosaponinの比色定量法

概要

論文の詳細を見る
A colorimetric method for determining saikosaponins in the range 10-60 μg has been described. Saikosaponins are warmed for 10 min at 70° with p-dimethylaminobenzaldehyde (1 mg) in glass-stoppered test tubes and again warmed for 30 min at 70° after addition of phosphoric acid (4 ml). The absorbances (y) of the reaction mixtures are recorded at 545 mμ. The quantities (x, fig) of the saponins are calculated as follows: a, x=212.179 y+0.424; c, x=333.556 y-0.567; d, x=179.533 y+0.013.
日本生薬学会の論文
1974-06-20