柴胡エキス中のSaikosaponinの定量(第3報) : Saikosaponinの抽出法

概要

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Saikosapoins were extracted from the powder of the crude drug "saiko" (from roots of Bupleurum falcatum L.) with methanol, n-butanol, water (pH 5.0 and 10.2), 2% KOH in water and 2% KOH in methanol. The highest yield of saponins a, c and d was in the 2% KOH-methanol extract. Saponin b was found only as a trace in the extract. Using this solvent, about 90% of the total saponins was obtained in the first extraction. Considerable amounts of saponin d were converted to ba when extraction took place for a long time or with acidic solvents. To determine the quantity of saponins in Bupleurum root, the material need only be extracted twice for one hour with 2% KOH-methanol under reflux. Quantities (x, μg) of saikosaponins b_1, b_2, b_3 and b_4 are calculated with the following formulas using absorbances at 545 nm (y) of reaction mixtures of the saponins with p-dimethylaminobenzaldehyde and phosphoric acid: for standard saponins, b_1 x=182.8157y+1.097 b_2 x=195.695y-0.037 b_3 x=207.469y+0.830 b_4 x=229.885y-0.680 for saponin bg recovered from thin-layer plates, x=214.133y-0.107.
日本生薬学会の論文
1975-12-20