柴胡エキス中のSaikosaponinの定量(第2報) : 薄層クロマトグラフィーによるSaponinの分離

概要

論文の詳細を見る
A thin-layer chromatographic separation method for determining quantities of saikosaponins contained in Bupleurum extract has been described. Each samples are spotted on two Silica Gel HR plates containing 13% gyps. Spots of mixtures of saikosaponin a and d are separated from those of b and c by development of a plate with chloroform-methanol-water (30 : 10 : 1). Spots of c and d are separated from those of mixtures of a and b by repeated development of another plate with ethyl acetate-ethanol-water (18 : 2 : 1). Each spots are detected by water spraying, marked, scraped from plates after dried, put into test tubes and treated with p-dimethylaminobenzaldehyde and phosphoric acid. The reaction mixtures are centrifugated and the absorbances (y) of the clear supernatants are recorded at 545 mμ. The quantities of the saponins (x, μg) are calculated as follows: a. x=235.128 y +1.646; b, x=366.838 y+0.209; d, x=198.531 y-0.198. Spots of saikosaponins and saikogenins on thin-layer plates are detected as pink spots by spraying 1% ethanolic solution of p-dimethylaminobenzaldehyde and sulfuric acid followed by heating.
日本生薬学会の論文
1974-06-20