2A2-A14 ガラス製リアクタを用いたリアルタイムPCR化学ICの開発

概要

論文の詳細を見る
In previous reports, we have developed a finger tip sized chemical device which can perform real-time PCR. In this paper, we report two major modifications. Firstly, we changed the reactor's material from PDMS to glass, drastically improving its chemical stability and performance. We used a femtosecond laser to selectively etch photosensitive glass fabricating a three-dimensionally structured reactor. Secondly, we incorporated the optical system that measure fluorescent intensity within the device. As a result, we have succeeded in greatly improving the efficiency of DNA amplification along with the realization of portability.