238 3次元振動ステージによる細胞培養コントロールデバイスの開発

概要

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In this study, a three-dimensional vibration stage is developed. This device provides the ability to simulate mechanical environment of the cells in vivo. Three piezoelectric ceramics are bonded in three orthogonal directions, so that the V-shaped vibration stage is able to mount a standard-sized culture dish and perform three-dimensional vibration up to around 80μm in each direction. Because of its simple structure, a sterilization treatment, which should be done before setting the vibration stage in a CO_2 incubator to avoid contamination, can be carried out without difficulty. Focusing on osteoblast's character, which is sensitive to external mechanical stimuli, a normal human osteoblast (NHOst) is stimulated by the developed three-dimensional vibration stage. In order to estimate effect of dynamic stimulation upon cultured cell, we define parameters of projected area and slederness ratio of the cultured NHOst. It is found that the vibration stimulation affects cell morphology.
2009-08-03