237 骨芽細胞のストレスファイバー制御と動的力学刺激の影響評価

概要

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In this study, a micro sensor-actuator device is developed to enforce dynamic stimulation and to detect changes of mechanical properties by the stimulation induced in a cultured adhesive cell. The micro device utilizes a clamped-free beam type vibrator of which the vibration is excited by a piezoelectric ceramic. The device has an ability to measure mechanical property of a cultured cell as compliance. It was mounted on a micromanipulator system, while dimensions and morphologies of the cells were measured by an inverted phase contrast microscope system. Experimental studies have been carried out by using cultured normal human osteoblast (NHOst), because of its sensitivity to external mechanical stimuli. Cytoskeletal condition was controlled by repolymerization time after depolymerization treatment with a use of Cytochalasin D. The method has shown enough capability to detect difference of mechanical properties due to changes of cytoskeletal stress fiber architecture in NHOst.
一般社団法人日本機械学会の論文
2009-08-03